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流式实验常用Protocols
时间：2017-12-27 21:52:03
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Protocols
Acquisition and Analysis
Procedure for Setting Compensation for Multicolor Flow Cytometric Analysis
The Escapee Phenomenon
Apoptosis
Annexin V with Cell Surface Antibody Staining for Suspension Cells
Annexin V Staining of Adherent Cells for Flow Cytometry
Annexin V Staining Protocol
APO-BRDU™ Procedure
APO-DIRECT™ Procedure
Induction of Apoptosis by Treatment with Camptothecin
Induction of Apoptosis by Treatment with Staurosporine
Receptor-Mediated Apoptosis in Mouse Cells Using Anti-Fas/CD95 (Clone Jo2)
Receptor-Mediated Apoptosis in Human Cells Using Anti-Fas/CD95 (Clone DX2)
Cell Cycle/DNA Analysis
Detection of BrdU Incorporation in DNA Synthesizing Cells
Flow Cytometry Staining Protocol for Detection of Ki-67
Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs
Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes
Staining Procedure for Flow Cytometric Detection of Human Cyclins
Cytokine (Intracellular Staining) Sample Preparation
Activation of Immune Cells for Cytokine Production
Cell Fixation/Permeabilization Kits for Intracellular Cytokine Analysis
Generation of Human IL-17–Producing Cells (Th17-like Cells) in Vitro
Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis
General
Bulk Erythrocyte Lysing with Ammonium Chloride for Flow Cytometry Immunophenotyping
Cell Surface Staining of Stem Cell Lines and Other Adherent Cells for Flow Cytometry
Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs)
Live Cell Discrimination and Measurement of Phosphorylation and Cell Surface Markers in Thawed and Activated Human PBMCs
Tissue Preparation for Surface Antigen Staining
Human Sample Preparation
Cell Surface Staining of Human PBMCs and Suspension Cell Lines
Direct Immunofluorescence Staining of Mononuclear Cells
Direct Immunofluorescence Staining of Whole Blood Using a Lyse/No-Wash Procedure
Direct Immunofluorescence Staining of Whole Blood Using a Lyse/Wash Procedure
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
Indirect Immunofluorescence Staining of Human Platelets
Indirect Immunofluorescence Staining of Mononuclear Cells
Isotype Control Antibody Selection Guide
Intracellular Staining of Human Red Blood Cells
Platelet Activation, Staining, and Analysis
Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs
Surface Staining of Human Red Blood Cells
Mouse/Rat Sample Preparation
Immunofluorescent Staining of Mouse and Rat Leukocytes
Preparation of Murine Splenocytes
The Uses of BD Fc Block in Immunophenotyping of Mouse or Rat Leukocytes
Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes
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