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产品简介
M5 Hiper Lipo2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells. DNA-Lipo 2000 complexes must be made in serum-free medium such as Opti-MEM® Reduced Serum Medium and can be added directly to cells in culture medium, in the presence or absence of serum/antibiotic. It is not necessary to remove complexes or change/add medium after transfection. The amount of Lipo 2000 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of Lipo2000 Reagent to determine an optimum amount.
产品组分
产品组分
MF135-01
MF135-02
M5 Hiper Lipo2000 Transfection Reagent
0.75 ml
1.5 ml
储存条件
长期保存，请置于4˚C (切勿结冰冻住)。
常见细胞的转染效率（仅供参考，实验条件不同转染效率会有差别）：
| 细胞种类 | HEK293 | HCT 116 | WRL-68 | HepG2 | NIH/3T3 | THP-1 | Hela | MCF-7 | 293T | TS cell | HO1980 | A549 |
| 转染效率 | >80% | >80% | ~80% | ~80% | ~80% | >50% | >80% | >80% | >80% | >60% | >60% | >80% |
| 细胞种类 | MEF | Chok1 | Hep3B | C2C12 | Neuro-2a | HUVEC | MDCK | Hep2C | WEHI | B50 | Calu1 | L929 |
| 转染效率 | >50% | >50% | >80% | >80% | >70% | >80% | >80% | >80% | >80% | >70% | >70% | >70% |
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